No, you only need to use 2 per size. Just make sure your media passes the requirements at the top of the column.
The top sieve, in your example the #50, cannot exceed 1/2% of the total sample weight remaining on the sieve after it has been run through the RoTap.
The bottom sieve (#35) cannot have more than 20% of the sample weight pass through. You can check for a minimum of 79 1/2% remaining on the sieve or insure no more than 20% of the total weight ends up in the bottom pan.
Note: The cases where 5 sieves are used is when inspecting NEW media other than cut wire.
